{"key":"acridine orange protocol zebrafish","title":["Using Acridine Orange to Measure Cell Death in Ethanol ...","Acridine Orange staining and visualization in zebrafish to ...","A rapid apoptosis assay measuring relative acridine orange ...","Analysis of Apoptosis in Zebrafish Embryos by Whole-mount ...","Histological Methods - Protocols - ZFIN Community Wiki","Developmental Apoptosis Mediates Entry and Positioning of ...","RESEARCH ARTICLE TECHNIQUES AND RESOURCES ...","Zebrafish Module - MBL","Acridine orange, programmed cell death assay in whole ...","Nestin Is Essential for Zebrafish Brain and Eye Development ...","Role of Programmed Cell Death in Defining Zebrafish ...","Designing experimental setup and procedures for studying ...","Activation of apoptosis and caspase\u20103 in zebrafish early ...","-1- Activation of Apoptosis and Caspase-3 in Zebrafish Early ...","Designing experimental setup and procedures for ... - CiteSeerX","Zebrafish as an in Vivo Screen for Early Black Cranberry ...","Zebrafish - Mary Ann Liebert, Inc.","Molecular insights and cell cycle assessment upon exposure ...","A versatile automated high-throughput drug ... - bioRxiv","Acridine Orange and Ethidium Bromide (AO\/EB) in ... - SciELO","Evaluation of the Developmental Toxicity Induced ... - Frontiers","Resveratrol Inhibits Oxidative Stress and Prevents ... - MDPI","Apoptosis in the Developing Zebrafish Embryo - Core","Origins and unconventional behavior of neutrophils in ...","Electroporation-based methods for in vivo , whole mount and ...","Mechanistic insight into ROS and neutral lipid alteration ...","Effects of Low-Dose Gamma-Ray Radiation on Apoptosis and ...","Acute Exposure to Permethrin Modulates Behavioral ...","In vivo micronucleus screening in zebrafish by flow cytometry ...","Acridine Orange\/Ethidium Bromide (AO\/EB) Staining to Detect ...","Infant and adult SCA13 mutations differentially affect Purkinje ...","Huntingtin function during zebrafish - Adelaide Research ...","Use of Zebrafish in Drug Discovery Toxicology | Chemical ...","Research Article - :: ALGAE","Distinct regulatory networks control the development of ...","CLONING, EXPRESSION AND FUNCTIONAL ANALYSIS OF ...","Fluorescent toxicity endpoints as promising tools to progress ...","Hydroxylated PBDEs induce developmental arrest in zebrafish","Comparative analysis of the toxicity of gold ... - Strathprints","In Situ Hybridization Protocols and Methods | Springer Nature ...","Prolactin functions as a survival factor during zebrafish ...","The zebrafish candyfloss mutant implicates extracellular ...","Essential Zebrafish Methods: Cell and Developmental Biology","Alcohol: Methods and Protocols","Distinct roles for BAI1 and TIM-4 in the engulfment of dying ...","Regulated Cell Death Part A: Apoptotic Mechanisms","The Zebrafish: Cellular and Developmental Biology, Part B","Developmental Biology Protocols","The Zebrafish: Cellular and Developmental Biology","Methods for detection and analysis of apoptosis signaling in ...","Confocal microscopy protocol for adherent cells","Reagents For Analyzing Autophagy - Autophagy | Sigma-Aldrich","Acridine orange *10 mg\/mL solution in water* | AAT Bioquest","Acridine orange - YouTube"],"href":["http:\/\/scholar.google.com\/scholar_url?url=https:\/\/www.liebertpub.com\/doi\/abs\/10.1089\/154065802761001293&hl=en&sa=X&ei=TlcCYPyRBaXcsQLzuYuIAg&scisig=AAGBfm0NcyFAsf57aJCegYDcEsyr5zL2cg&nossl=1&oi=scholarr","http:\/\/scholar.google.com\/scholar_url?url=http:\/\/psulibrary.palawan.edu.ph\/wtbooks\/resources\/pdf\/910799.pdf","http:\/\/scholar.google.com\/scholar_url?url=https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2186061\/&hl=en&sa=X&ei=TlcCYPyRBaXcsQLzuYuIAg&scisig=AAGBfm2GvMHiB0nZXvfZVSVXY3gQm9vYZw&nossl=1&oi=scholarr","https:\/\/www.biotek.com\/resources\/application-notes\/using-acridine-orange-to-measure-cell-death-in-ethanol-treated-zebrafish-embryos\/","https:\/\/www.researchgate.net\/post\/Acridine_Orange_staining_and_visualization_in_zebrafish_to_study_apoptosis2","https:\/\/pubmed.ncbi.nlm.nih.gov\/18041929\/","https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4109746\/","https:\/\/wiki.zfin.org\/display\/prot\/Histological+Methods","https:\/\/www.cell.com\/cms\/10.1016\/j.celrep.2016.06.033\/attachment\/7bd2a8df-5254-4d8f-9087-c18b65b4efdf\/mmc1","https:\/\/dev.biologists.org\/content\/early\/2016\/10\/08\/dev.122721.full.pdf","http:\/\/mblembryology.stowers.org\/Images\/Exp Systems\/LAB MANUALS 2010\/ZebrafishManual2010.pdf","https:\/\/link.springer.com\/content\/pdf\/bbm:978-1-59259-270-8\/1.pdf","https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0009318","https:\/\/digitalcommons.liberty.edu\/cgi\/viewcontent.cgi?article=1064&context=fac_dis","http:\/\/www.cityu.edu.hk\/phy\/nru\/pub_j216.pdf","https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/full\/10.1002\/dvdy.20124","https:\/\/citeseerx.ist.psu.edu\/viewdoc\/download?doi=10.1.1.838.2403&rep=rep1&type=pdf","http:\/\/citeseerx.ist.psu.edu\/viewdoc\/download?doi=10.1.1.704.8439&rep=rep1&type=pdf","https:\/\/www.scirp.org\/pdf\/ajmb_2014032813520884.pdf","https:\/\/www.liebertpub.com\/doi\/pdfplus\/10.1089\/154065802761001293?src=recsys","https:\/\/www.nature.com\/articles\/s41598-020-64157-3","https:\/\/www.biorxiv.org\/content\/10.1101\/2020.12.16.423108v1.full.pdf","https:\/\/scielo.conicyt.cl\/scielo.php?script=sci_abstract&pid=S0717-95022014000100014&lng=en&nrm=iso","https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2020.00032\/full","https:\/\/www.mdpi.com\/1422-0067\/21\/11\/3838\/pdf","https:\/\/core.ac.uk\/download\/pdf\/82232322.pdf","https:\/\/ashpublications.org\/blood\/article\/111\/1\/132\/107908\/Origins-and-unconventional-behavior-of-neutrophils","https:\/\/neuraldevelopment.biomedcentral.com\/articles\/10.1186\/1749-8104-2-6","https:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2018\/tx\/c7tx00300e\/unauth","https:\/\/meridian.allenpress.com\/radiation-research\/article\/194\/1\/61\/436354\/Effects-of-Low-Dose-Gamma-Ray-Radiation-on","https:\/\/www.hindawi.com\/journals\/omcl\/2019\/9149203\/","https:\/\/academic.oup.com\/mutage\/article\/31\/6\/643\/2468955","http:\/\/europepmc.org\/abstract\/MED\/22485874","https:\/\/elifesciences.org\/articles\/57358","https:\/\/digital.library.adelaide.edu.au\/dspace\/bitstream\/2440\/62458\/8\/02whole.pdf","https:\/\/pubs.acs.org\/doi\/10.1021\/acs.chemrestox.9b00335","https:\/\/www.e-algae.org\/m\/journal\/view.php?number=2919","http:\/\/www.bloodjournal.org\/content\/129\/4\/509.full?sso-checked=true","https:\/\/etd.ohiolink.edu\/apexprod\/rws_etd\/send_file\/send?accession=osu1052847984&disposition=inline","http:\/\/publications.rwth-aachen.de\/record\/481055\/files\/481055.pdf","https:\/\/cpb-us-w2.wpmucdn.com\/sites.baylor.edu\/dist\/b\/55\/files\/2014\/11\/Hydroxylated-PBDEs-induce-developmental-arrest-in-zebrafish-2ar87o9.pdf","https:\/\/strathprints.strath.ac.uk\/63720\/1\/Patibandla_etal_JAT_2018_Comparative_analysis_of_the_toxicity_of_gold_nanoparticles_in_zebrafish.pdf","https:\/\/experiments.springernature.com\/techniques\/in-situ-hybridization","http:\/\/core.ecu.edu\/biol\/zhuy\/publications\/2009Nguyen.pdf","https:\/\/www.pnas.org\/content\/104\/17\/7092","https:\/\/www.zora.uzh.ch\/id\/eprint\/183595\/1\/ncomms5046.pdf","https:\/\/lab.research.sickkids.ca\/derry\/wp-content\/uploads\/sites\/11\/2015\/08\/Lant-Derry-2013.pdf","http:\/\/desijawani.com\/arduino-button-kf5iq\/confocal-microscopy-protocol-for-adherent-cells.html","https:\/\/www.sigmaaldrich.com\/life-science\/cell-biology\/cell-biology-products.html?TablePage=104899414","https:\/\/www.aatbio.com\/products\/acridine-orange-10-mg-ml-solution-in-water","https:\/\/www.youtube.com\/watch?v=03VktMbMYvg"],"desc":["Zebrafish embryos were treated with ethanol during the first 24 hours of development and the effects of ethanol treatment on cell death was ...","I'm trying to use Acridine Orange (A.O) staining method to obtain qualitative data. ... I stain the zebrafish larva in 5 ug\/ml A.O in E3 media for 20 minutes and wash it in E3 ... (P.S: that protocol was originally used for lysosomal stability assay).","A rapid apoptosis assay measuring relative acridine orange fluorescence in zebrafish embryos. Zebrafish. Summer 2007;4(2):113-6. doi: 10.1089\/zeb.","Compared to acridine orange staining, which can identify apoptotic cells in ... The protocol described herein uses an antibody that specifically ...","... A Simple Whole-Mount Staining Protocol For Bone And Or Cartilage ... Histological Results With Zebrafish Embryos \u00b7 Zebrafish Monoclonal ... Visualization of dead cells with Acridine Orange \u00b7 Antibody staining on Sections.","in the Zebrafish Brain ... F, I) Acridine orange staining in pU.1-morphant embryos. Dotted ... (Millipore) was used following the protocol suggested by the provider.","application protocols in zebrafish (Hans et al., 2009; Kroehne et al., 2011; ... 10  g\/ml acridine orange (Sigma Aldrich)\/30% Danieau and ...","Some helpful hints for working with zebrafish embryos! ... Alternative wholemount antibody labeling protocol . ... Acridine Orange staining .","Acridine orange, ... zebrafish mutant screening, 455. Agarose gels, DNA purification for microinjection, materials, 78 overview, 77, 78 protocol, 79, SO.","Methodology\/Principal Findings As nestin is conserved in zebrafish and its ... assay and acridine orange staining and proliferation by BrdU incorporation, pcna ... The zebrafish experimental protocols were approved by IACUC ...","mjcroscopy protocol. E. Total cell death assay using Acridine Orange (AO). 30. F. Dual staining using 4', 6 Diamidino-2-phenylindole. 30. (DAPI)\/ AO mix.","The levels of apoptosis in irradiated zebrafish embryos at 24 hpf were quantified through staining with the vital dye acridine orange, followed by counting the stained cells ... usual protocol of induction of adaptive response in cell culture is.","Thus, by many criteria, cell death in zebrafish is apoptotic; many of the markers ... Acridine orange (AO) exhibits metachromatic fluorescence that is ... The TUNEL assay was adapted from established protocols (Gavrieli et al., ...","Stock Acridine orange (AO) and Propidium iodide (PI) (Calbiochem) were prepared in ERM to a final working concentration of 100\u00b5g\/ml. Embryos were collected, ...","Acridine orange apoptosis in- dicator dye was incorporated into the treatment protocol, and it was observed that irregular epi- thelial cell death ...","Zebrafish embryos were immersed in 1 mg\/ml acridine orange (acridinium chloride hemi[zinc chloride]) in fish water for 30 min and rinsed thoroughly twice in 10 ml ...","Apoptotic assay using acridine orange staining revealed that apoptosis (as green dots) was induced less in the zebrafish exposed to higher ...","embryos, and the WiSoft\u00ae Athena Zebrafish Application for analysis, which harnesses an Artificial ... acridine orange for use in toxicity screens. ... Acquisition and analysis protocols can be customised and then saved, allowing ...","Excretion Product of Shigella dysenteriae (SdyEP) Induced Cell Death in Early Larval Stage of Zebrafish (Danio rerio): Acridine Orange and Ethidium Bromide (AO ...","The hatching rates and body length of the zebrafish larvae was ... Generation of ROS was examined at 72 hpf according to protocol of the ROS assay kit ... Apoptotic cells were detected by acridine orange (AO) staining in live ...","Resveratrol Protects Zebrafish Embryos form ZnO NP-Induced Mortality ... in zebrafish, the acridine orange\/propidium iodide (AO\/PI) assay was ... All the assays complied with animal protocol guidelines required by the Qatar ...","cells during normal development of the zebrafish embryo from 12 to 96 h postfertilization. ... with acridine orange in living embryos in our lab (data not shown).","In adult zebrafish, mpx was found to be expressed in neutrophils of the kidney5,7 (the ... that does not correlate with increased apoptosis, as revealed by acridine orange vital staining (data not shown). ... Protocol for FITC tyramide synthesis.","We present a new protocol for zebrafish brain electroporation. ... by staining with acridine orange, in electroporated embryos (Figure 2e).","Apoptosis was induced in embryonic zebrafish exposed to synthesized TiO2 nanoparticles with the help of the acridine orange staining protocol mentioned by ...","Zebrafish embryos are highly sensitive to ionizing radiation in the early ... accordance with the protocol for the mRNA-Seq Sample Preparation Kit ... Zebrafish apoptosis detection by using acridine orange fluorescent method.","The exploratory behavior followed the established protocols using zebrafish ... The fluorescent dye Acridine Orange (AO) was used to detect apoptotic cells.","At this step of the protocol, the zebrafish samples contained in ... dried overnight and stained with 40 \u00b5L of acridine orange (0.1% in PBS).","Acridine Orange\/Ethidium Bromide (AO\/EB) Staining to Detect Apoptosis. ... CSH Protocols, 01 Aug 2006, 2006(3) ... Inhibits Oxidative Stress and Prevents Mitochondrial Damage Induced by Zinc Oxide Nanoparticles in Zebrafish (Danio rerio).","Zebrafish were then washed three times for 5 min in fish water lacking acridine orange. The staining and washing protocol was performed under ...","Detection of apoptosis in whole embryos was assessed by staining with the vital dye, acridine orange (AO) [132]. The acridine orange protocol was adapted from ...","The number of end points that are evaluated in the currently used zebrafish embryo protocols is much more limited compared to the exhaustive ...","Furthermore, in vivo studies of the SA extract in zebrafish showed ... observed, followed by the protocol of \u201cmeasurement of AAPH-induced intracellular ROS ... Cell death was observed in zebrafish embryos by acridine orange ...","Zebrafish were maintained according to standard protocol. ... Acridine orange and terminal deoxynucleotidyltransferase-mediated dUTP nice end labeling ...","Acridine orange staining of zebrafish embryos\u2026\u2026\u2026\u2026\u2026\u2026194. In situ TUNEL assay ... cycling protocol consisted of one cycle for 3 min at 94oC followed by 35 ...","To date, the application of the zebrafish (Danio rerio) embryo toxicity test ... Centre for Validation of Alternative Methods (ECVAM) published a protocol to promote the ... commonly performed by staining with the vital dye acridine orange (AO) ...","Exposure protocols were similar to those used in the acridine orange assay. After four hours of exposure, embryos were fixed in 4% parafor-.","Keywords Nanoparticles; nanospheres; nanorods; zebrafish; toxicity. 39. 40 ... (TEM) and acridine orange staining have been used to investigate the distribution of. 88 ... The protocol was carried out in 20\u00b5l reaction volume in two steps.","High-resolution in situ hybridization to whole-mount zebrafish embryos ... The TUNEL and acridine orange methods are used to detect dead or dying cells in a ...","mechanisms of apoptosis appear to be conserved between zebrafish and late ... caspase-3 immunostaining, or acridine orange staining. Embryos ... protocol described in the previous paragraph and blocked in 5% goat.","At 60 hpf, embryos were injected with acridine orange, and severe fiber detachment was induced by using the anesthetic recovery protocol ...","Apoptosis Detection by Acridine Orange Another method of apoptotic cell detection that can ... Protocol 1. 2. ... C. In Situ Hybridization RNA expression analysis by in situ hybridization of antisense probes in whole-mount zebrafish embryos is a ...","Methods and Protocols Laura E. Nagy ... Because the acridine orange assay is not absolutely specific, as non apoptotic cells can ... The TUNEL analysis is more specific for the detection of apoptotic cellular death in whole zebrafish embryos.","neuronal cell death progression in embryonic zebrafish brains and studied the ... Caspase-3, and Acridine Orange (AO) that stains condensed ... BAI1 probe following the protocol described by Ober and Schulte-Merker34.","The following protocol describes how to remove zebrafish embryos from their protective chorions and stain apoptotic cells using acridine orange. 2.1.1 Required ...","Protocol: 1. ... Apoptosis Detection by Acridine Orange Another ... of apoptotic cell detection that can be performed on living embryos is acridine orange staining.","In organisms like zebrafish, chick, and mouse, as well as Drosophila, ... Acridine Orange Staining One of the simplest and most rapid techniques to examine ...","Protocol: 1. 2. Live dechorionated embryos are incubated in a 2 g\/ml solution of acridine orange (Sigma, St. Louis, MO) in 1 \u00c2 PBS for 30 m at room temperature.","protocol with a CED-3 preparation [32]. Although there is varying ... as acridine orange (AO) and SYTO-12 facilitate quantification of apoptotic nuclei in the ...","confocal microscopy protocol for adherent cells This protocol describes how to ... the method is based on intrinsic fluorescence properties of acridine orange (AO), ... Stitched image of a 4 day old Zebrafish embryo with labeled endothelial cells.","The vital dyes Acridine Orange and Dansylcadaverine (MDC) are commonly used to study autophagy. Acridine Orange is a lysotropic dye that accumulates in ...","Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell ... experimental protocol for appropriate experimental\/physiological buffers."],"related":["acridine orange live cell staining protocol","acridine orange apoptosis","orange zebrafish","zebrafish immunofluorescence protocol","orange zebrafish breeding","acridine orange\/ethidium bromide (ao\/eb staining principle)","acridine orange\/ethidium bromide (ao\/eb) staining to detect apoptosis","alizarin red staining protocol zebrafish"],"ask":[],"strong":["zebrafish","acridine orange","protocol","protocols","acridine orange protocol"]}